Chapter 2 Microscopy
2.2 Bright-Field (BF) Microscopy
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2.2 Bright-Field (BF) Microscopy
Bright-field (BF) microscopy workflow
Outline: Remove all unnecessary optical elements from the optical path. Focus and center the
condenser. Adjust the aperture diaphragm for a better image.
1
Reset convenient functions. ¨ Page 31
2
Turn on the dia illuminator. ¨ Page 31
3
Adjust the illumination for optimal color reproduction. ¨ Page 32
4
Prepare the optical path. ¨ Page 32
5
Set up for bright-field microscopy. ¨ Page 33
7
Adjust the diopters and the interpupillary distance. ¨ Page 34
8
Re-adjust the focus. ¨ Page 34
9
Center the condenser. ¨ Page 35
10
Observe the specimen. ¨ Page 36
11
Change the specimen. ¨ Page 37
12
End the observation. ¨ Page 37
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