Chapter 2 Microscopy
2.1 Introduction to Microscopy
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Sample configuration
This section describes the microscopy procedures under the assumption that the following devices are
attached to the microscope.
B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS
OFFSET
OUT
DI
CHROI
C
M
I
RROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z
-
RESET
1X
1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
PFS
DOWN
UP
PFS
OFFSET
CONTROLLER
FINE
COARSE
Figure 2-1 Sample configuration
1. Ti-E microscope body
2. AC adapter, power cord *
3. TI-T-B Eyepiece Base Unit
4. TI-TD Eyepiece Tube B
5. CFI 10x eyepiece (x2)
6. TI-DH Dia Pillar Illuminator 100W,
filters (NCB11, ND4, GIF)
7. D-LH/LC Precentered Lamphouse LC,
12V 100W Halogen Lamp
8. TI-PS100W Power Supply, power cord
9. TI-C Condenser Turret,
LWD Condenser Lens,
condenser cassette for bright-field
microscopy,
condenser cassette for phase contrast
microscopy
10. TI-SR Rectangular Mechanical Stage
11. TI-ND6-PFS PFS Motorized Nosepiece,
PFS Offset Controller,
10x and 40x objectives,
phase contrast objective
* “2. AC adapter, power cord” not shown in Figure 2-1.
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5
6
7
8
10
11
1
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