Chapter 2 Microscopy
2.4 External Phase Contrast Microscopy
44
Selecting objectives and phase plates
To perform external phase contrast microscopy, you will need to use the TI-T-BPH External Phase Contrast
Eyepiece Base Unit.
You will also need to select a phase plate that is suitable for the objective being used, and attach it to the
eyepiece base unit. Refer to the following table to select an objective and an appropriate phase plate.
Up to three phase contrast plates (A, B, and C) can be attached to the TI-T-BPH External Phase Contrast
Eyepiece Base Unit.
Condenser phase contrast code
(Supported condenser lens)
Objective
External phase
contrast ring
1 Ph3 (LWD, CLWD) P Apo 60x WI (NA1.2) 60x/Ph3
2 Ph3 (LWD, CLWD) P Apo VC 60x WI (NA1.2) 60x/Ph3
3 Ph3 (LWD, CLWD) P Apo VC 60x H (NA1.4) 60x/Ph3
4 Ph4 (CLWD) Apo TIRF 60x H (NA1.49) 60x/Ph4
5 Ph4 (CLWD) P Apo TIRF 60x H (NA1.45) 60x/Ph4
6 Ph3 (LWD, CLWD) P Apo VC 100x H (NA1.4) 100x/Ph3
7 Ph4 (CLWD) Apo TIRF 100x H (NA1.49) 100x/Ph4
External phase contrast microscopy workflow
Outline: Place the objective (refer to the table above), the condenser lens, and the condenser
cassette into the optical path. Center the positions of the eyepiece base unit (TI-T-BPH) and the
ring in the condenser cassette.
1
Adjust the focus onto the specimen with BF microscopy. ¨ Page 45
2
Center the external phase contrast ring. ¨ Page 45
3
Center the condenser annular diaphragm. ¨ Page 46
4
Observe the specimen. ¨ Page 46
5
Change the objective. ¨ Page 47
6
Change the specimen. ¨ Page 47
7
End the observation. ¨ Page 48
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