Olympus IX2-DSU - Page 27

DETAILS OF EACH OPERATION / Selection of fluorescent mirror unit
II . DSU
II . 3-3
Page
Table of combination for mirror unit and dichroic mirror filter
Excitation
Method
Mirror Unit Dichroic Mirror
Excitation
Filter
Barrier Filter Fluorochrome
U-MWU2 BP330-385
U
U-MNU2
DM400
BP360-370
BA420
• Auto fluorescence observation
• DAPI Dyeing: DNA
• Hoechest 33258, 33342 Dyeing:
Chromosome
V U-MNV2 DM455 BP400-410 BA455
• Catecholamine observation
• serotonin observation
• tetracycline Dyeing: Bone, Teeth
U-MWBV2 BP400-440
U-MNBV2
DM455
BP420-440
BA475
BV
U-MCFPHQ DM450 BP425-445 BA460-510
• quinacrine, quinacrine mustard
Dyeing: Chromosome
• thioflavine S Dyeing: lymph cell
• acriflavine Dyeing: Nucleic acid
• CFP use
U-MGFPHQ DM485 BP460-480 BA495-540
U-MWB2 BP460-490
U-MNB2 BP470-490
B
U-MSWB2
DM500
BP420-480
BA520IF
U-MWIB2 BP460-490
U-MNIB2
DM505
BP470-490
BA510IF
IB
U-MYFPHQ DM505 BP490-500 BA515-560
• FITC Dyeing: Fluorescent
antibody test
• acridine orange Dyeing:DNA, RNA
• auramine Dyeing: TB bacteria
• EGFP, S65T, RSGFP use
• YFP use
U-MWG2 BP510-550
U-MNG2 BP530-550G
U-MSWG2
DM570
BP480-550
BA590
DSU-
MRFPHQ
DM560 BP535-555 BA570-620
IG
U-MWIG2 DM565 BP520-550 BA580IF
• rhodamine, TRITC Dyeing:
Fluorescent antibody test
• propidium iodide Dyeing: DNA
• RFP use
IY U-MWIY2 DM600 BP545-580 BA610IF
texas red Dyeing: Fluorescent
antibody test