PerkinElmer EnVision User Manual

Specifications
74
TRF
DELFIA
100 55 s 2 m 32 s 8 m 52 s
AlphaScreen
Std
- 1 m 32s 4 m 54 s 19 m 28 s
AlphaScreen
HTS
- 51 s 1 m 52 s 9 m 23 s
Measurement Performance
Technology Feature Performance
Fluorescence
intensity, filters
Detection limit (96-
plate, 200 µL)
< 4 pM, <0.8 fmol/well,
fluorescein, 100 flashes from top
Fluorescence
intensity, filters
Detection limit
(384-plate, 50 µL)
< 4 pM, <0.2 fmol/well,
fluorescein, 100 flashes from top
Fluorescence
intensity, filters
Detection limit
(1536-plate, 7.5
µL)
< 20 pM, <0.15 fmol/well,
Fluorescein, 100 flashes from top
Fluorescence
intensity,
monochromator
Detection limit (96-
plate, 200 µL)
< 10 pM, <2 fmol/well,
Fluorescein, 100 flashes from top
Fluorescence
intensity,
monochromator
Detection limit
(384-plate, 50 µL)
< 80 pM, <4 fmol/well,
Fluorescein, 100 flashes from top
Fluorescence
intensity,
monochromator
Bandwidth
(Excitation /
Emission)
< 8nm
Fluorescence
polarization
Detection limit (96-
plate, 200 µL)
< 3 mP, 1nM Fluorescein, 100
flashes
Fluorescence
polarization
Precision (384-
plate, 50 µL)
< 3 mP, 1nM Fluorescein, 100
flashes
Fluorescence
polarization
Precision (1536-
plate, 7.5 µL)
< 7 mP, 1nM Fluorescein, 100
flashes
Time-resolved
fluorescence
Detection limit (96-
plate, 200 µL)
< 55 fM, Eu, <11 amol/well, 100
flashes from top
Time-resolved
fluorescence
Detection limit
(384-plate, 50 µL)
< 55 fM, Eu, < 2.8 amol/well, 100
flashes from top

PerkinElmer EnVision Specifications

General

Light Source	Xenon flash lamp
Software	EnVision Manager
Read Modes	Absorbance, Fluorescence, Luminescence, Time-Resolved Fluorescence, Fluorescence Polarization
Plate Formats	384-well
Detection Technology	PMT
Temperature Control	Ambient to 45°C