PerkinElmer EnVision User Manual

Specifications
75
Technology Feature Performance
Time-resolved
fluorescence
Detection limit
(1536-plate, 7.5
µL)
< 150 fM, Eu, < 1 amol/well, 100
flashes from top
Linearity 4.5 decades, Eu
Absorbance
Filter /
Monochromator
Measurement range
(96-plate, 200 µL)
0-4 OD @ 405 nm
Absorbance
Filter /
Monochromator
Measurement range
(384-plate, 50 µL)
0-4 OD @ 405 nm
Accuracy @ 2 OD < 2% @ 405 nm
Precision @ 2 OD < 0.1 % @ 405 nm
Absorbance
Filter /
Monochromator
Measurement range
(1536-plate, 7.5
µL)
0-3 OD @ 405 nm
Absorbance
Monochromator
Bandwidth <8 nm
Absorbance
Monochromator
Wavelength
accuracy
±2.0 nm
Absorbance
Monochromator
Wavelength
precision
±0.2 nm
Luminescence Lower limit of
detection (96-plate,
200 µL)
< 75 pM, ATP, 1 second
Ultra Sensitive
Luminescence
Lower limit of
detection (384-
plate, 50 µL)
< 10 pM, ATP, 1 second
AlphaScreen
(Std and HTS)
Detection limit
(384-plate, 50 µL)
(25 µL,
phophorylated bio-
peptide, kinase
assay
< 100 amol

PerkinElmer EnVision Specifications

General

Light Source	Xenon flash lamp
Software	EnVision Manager
Read Modes	Absorbance, Fluorescence, Luminescence, Time-Resolved Fluorescence, Fluorescence Polarization
Plate Formats	384-well
Detection Technology	PMT
Temperature Control	Ambient to 45°C