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PicoQuant MultiHarp 150 - Page 67

PicoQuant MultiHarp 150
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PicoQuantGmbH MultiHarpSoftwareV.3.1.0.0
TTTR Real–Time Correlator
OpenstheTTTRmodereal-timecorrelatordialogbox.UsethisdialogforFCSpreviewduringaTTTRmode
run.Makesureallothermeasurementparametershavebeensetandtestedininteractivemodebeforeentering
TTTRcorrelatormode.Changingtothismodetakesafewsecondsforreconfigurationofthehardware.See
alsosection5.3.7.
Monochromator / TRES
Inorderto measureTime-Resolved Excitation/EmissionSpectra(TRES),the MultiHarp softwareprovidesa
TRESmeasurementmode,thatallowstocontrolamonochromatorviasteppermotorsandautomatedcollection
ofspectrallyresolvedlifetimehistograms.Clickingthisbuttonwilllaunchthedialogformanualmonochromator
controlandTRESsetup.Thereyoucansetparameterssuchasthestart,stepping,andendofthewavelength
scan.Therewillbeappropriateerrormessagesifthemonochromator/stepperandassociateddriversarenot
configuredproperly.
General Settings
Opensthesoftwaresettingsdialogbox.UsethisdialogtochangestandardsettingsoftheMultiHarpsoftware.
Notablytheseare:Display rate(0.1to1s),Draw mode(lines,stairs),Grid checkbox,Prompt overwrite(warning
beforeoverwritingexistingdata),selectivedisablingofWarningsandTTTR Marker Settings.Thecontrolcon-
nectoroftheMultiHarpprovidesTTLinputsforsynchronizationsignals.Themarkerscanbeenabledordis-
abledaswellasrecordedateithertherisingorfallingedgeofthecorrespondingTTLsignal.Theactiveedges
canbechosenhere.Thereisalsoaprogrammablehold-offtimetosuppressglitchesonthemarkersignals.
Thedialogalsoallowsselectivedisablingofwarningsthatyoudonotwishtoreceive.Allsettingswillbekeptin
theWindowsregistryandwillberetrievedatthenextprogramstart.TheyarestoredindividuallyforeachWin-
dowsuserprofile.
White Rabbit Settings
OpenstheWhiteRabbitdialog.UsethisdialogtoconfigureandestablishaWhiteRabbitlinkforclocksynchro-
nization.WhiteRabbitallowstimetransferandsynchronizationoverfiberbasedEthernetwithsub-nsaccuracy
(formoreinformationsee https://en.wikipedia.org/wiki/The_White_Rabbit_Project).Note,however,thatWhite
Rabbitsupportisstillbasic,tosomeextentsimplybecauseWhiteRabbitassuchisstillevolving.Currentlythe
MultiHarp supportsonlybasic WhiteRabbit functionality,notablyclocksynchronizationandtimetransferbe-
tweentwoMultiHarpdeviceswhereonedeviceisWhiteRabbitmasterandtheotherisWhiteRabbitslave.Sim-
ilarlysomeotherWhiteRabbitdevicesrunningasamastercanactastheclocksourceoftheMultiHarporthe
MultiHarpcanactasamasterandclocksourceforsomeotherWhiteRabbitdevice.Notallofthishasyetbeen
tested.WhiteRabbitsupportisexpectedtobeimprovedwithfuturesoftwareandfirmwareupdates.Pleasecon-
tactuswithyourrequirementsifyouareinterestedinimprovementsorextensionsofthisproductfeature.
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