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1. Press the CAL on the main page, then choose Fluorescein and press the OK key
to launch the fluorescein calibration page.
2. Follow the message prompts, insert the DI blank into the sample vial compartment
and press the OK key.
3. Follow the message prompts, and insert the 20 ppb standard into the sample vial
compartment and press the OK key.
4. Press any key to return to the main page.
Figure 9 Fluorescein Calibration
If calibration fails, the followings should be checked:
• The DI blank is being contaminated.
• The 20 ppb fluorescein standard solution is decayed or being contaminated.
• The light shield cover is not in the closing position.
• The sample vial compartment is blocked with debris, water, or other materials.
4.3 Fluorescein calibration (Firmware version v1.0r295 and after)
Deionized water (DI) as the blank calibration solution, the 50 ppb fluorescein, the 250
ppb fluorescein and the 500 fluorescein calibration standard solutions are needed.
1. Press the CAL on the main page, then choose Fluorescein and press the OK key
to launch the fluorescein calibration page.
2. Follow the message prompts, insert the DI blank into the sample vial compartment
and press the OK key.
3. Insert the 50 ppb standard into the sample vial compartment and press the OK key
to complete the low range calibration.
4. Press the OK key to proceed with middle range calibration or press any other keys
to return to main page.
5. Insert the 250 ppb standard into the sample vial compartment and press the OK
key to complete the middle range calibration.
6. Press the OK key to start proceed with high range calibration or press any other
keys to return to main page.
7. Insert the 500 ppb standard into the sample vial compartment and press the OK
key to complete the high range calibration.
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