17
The middle range and high range calibrations from steps 4 to 8 are optional if only low
range fluorescein measurement is intended.
Figure 10 Low Range Fluorescein Calibration
Figure 11 Middle Range Fluorescein Calibration
Figure 12 High Range Fluorescein Calibration
The standard solutions shall be stored in a brown or black opaque bottle. Exposing the
fluorescein standard to light will cause the standard losing the fluorescein
concentration. Many substances, such as quaternary amine cause a negative
interference. Many other substances such laundry detergents that contain optical
brightener will cause a significant positive interference.
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