REPLIGEN Spectrum KR2i User Manual

42 400-12355-000 Rev. 03
KR2i / KMPi TFF Systems
System Functionality
On both the Extra Feed Reservoir and Diafiltration Buffer Reservoir, install a vent. Ensure that
Auxiliary Pump 1’s head is closed on the tubing between the Diafiltration Buffer and Feed Reservoir.
The result of the setup is that the vacuum will pull the entirety of the Extra Feed Reservoir’s
contents as volume is depleted from the Process Reservoir the C function of C/D Mode. Once the
contents of the Extra Feed Reservoir are depleted, the vacuum will pull air. After the Concentration
set-point is reached (Conc Factor 1), the TFF system will then switch to D mode until the Diafiltration
set-point (Dia Volumes 1) is reached.
The TFF system will be able to properly calculate the Concentration Factor because it will know that
a Fed-Batch Concentration is taking place when the Permeate Scale increases while the Feed Scale
weight stays constant.
Starting Weight 20 kg
Conc Factor 1 10X Perm Wt After C1 18 kg
Feed Wt After C1
2 kg
Diaf Volumes 1 7 Diaf Wt 1 14 kg
Perm Wt After D1 32 kg
Feed Scale Permeate Scale
1. Feed Line to filter
3. Buffer Line
4. Vent Line
2. Retentate Line from filter

REPLIGEN Spectrum KR2i Specifications

General

Brand	REPLIGEN
Model	Spectrum KR2i
Category	Laboratory Equipment
Language	English