3. Assemble TFF flowpath and place Conductivity/Temp and/or UV in-line sensors at the
correct placement in the flowpath.
Conductivity Sensor: The sensors can be placed either in the permeate line or recirculation
line when used for the Diafiltration endpoint control.
NOTE: Conductivity sensors need to be filled with the initial starting buffer prior to starting the
automated sequence. When sensor is placed on permeate line this buffer should be the
same buffer that sample is in. This will eliminate any potential early shutdown of the
auto mode.
If the conductivity setpoint is lower than the starting value the system will stop the
sequence when the lower value is reached. If the conductivity setpoint is higher than the
starting setpoint then the system will stop the sequence at the higher value.
The system will only track in the linear range either positive or negative from the starting
conductivity.
UV Sensor: For UV alarms the sensors are usually placed on the permeate line to detect
sample breakthrough.
For UV Concentration automated control sequences, the sensors are usually placed in the
recirculation loop. The endpoint should be higher than the starting Absorbance.
For UV Dialfiltration automated control sequences, the sensors are usually placed in the
permeate line and the endpoint should be lower than the starting Absorbance as the protein
or other sample is washed through.
Place the tubing through the plunger mechanism by lifting up on the body of the valve and
fitting the tubing between the metal bar and the white plastic plunger. The body of the
valve can then be turned 180 deg to close of the opening by using the longer stainless steel
rods or let the tubing opening remain accessible with the shorter rods.
NOTE: Fill UV inline sensor with buffer and press the physical "Tare" button on UV box prior to
starting the sequence. For UV Diafiltration control on the permeate line the molecule of
interest should be detected in the cell prior to starting the automated sequence.
NOTE: Do not place heavy weight on top of UV fiber optic cables; do not fold UV fiber optic cables;
fiber optic cables are very fragile.
4. Connect all sensor(s) to corresponding port(s).
5. After making all connections, wait at least one minute for KONDUiT to establish connection
with TFF System
6. OPTIONAL: Launch KF Comm Data Collection workbook for TFF System
7. KONDUiT can be placed behind the KR2i or KMPi system. No physical buttons or interfaces
are necessary on the base unit. The UV photometer has a tare button that needs to be
accessible. Cables can be placed in the cable boxes provided with the KR2i or KMPi systems.
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