APPLICATIONS MANUAL
A.6 Revised 10/02/2012
Do’s and Don’ts
• DO leave breast or fatty tissue in Pre-Processing
Solution 30 to 60 minutes to firm tissue prior to trim-
ming.
• DO NOT use blue sponges for processing of tissue.
• DO use embedding bags, lens paper, etc.
• DO NOT process tissue for RNA studies with
Formalin-fixed tissue or solutions that have been
used for Formalin-fixed tissue.
• DO run RNA tissue after the processor is cleaned and
solutions are all changed.
• DO NOT process tissue with O.C.T. compound (used
for Cryotomy) residue in the Tissue-Tek Xpress x120.
• DO remove all O.C.T. compound (used for Cryotomy)
from specimens by rinsing with water prior to placing
in the Tissue-Tek Xpress x120.
• DO NOT process tissue thicker than 2 mm in
thickness on the Standard program.
• DO process tissue that is more than 3 mm in
thickness using the Extended program.
• DO NOT allow the faced tissue blocks to soak too
long in water.
• DO face the block and allow the block to soak/or cool
only for a short period of time.
• DO NOT use metal cassette lids.
• DO use plastic lids and remove metal staples and
sutures from tissue prior to placement in the cassette.
• DO NOT top off reagents or rotate reagents.
• DO change the reagents and solution after 1,500
cassettes or every 3 days, whichever occurs first.
• DO seal blocks after Microtomy with a thin layer of
paraffin if block will later be used for molecular stud-
ies
Disposal of Tissue-Tek Xpress
x120 Processing Solutions
Refer to MSDS sheets. Disposal should be in accord-
ance with Local, State and Federal regulations.