Sakura Tissue-Tek Xpress x120 User Manual

Revised 10/02/2012 A.7
Tissue-Tek Xpress x120 Processing Flow Chart for Standard Program
Tissue-Tek Xpress x120 Processing Flow Chart for Extended Program
NOTES:
• As needed, tissue difficult to section, can be im-
mersed in PPS for 15 minutes to firm up.
• Some immersion times can be significantly reduced
for small biopsies. The above baseline procedure
creates optimal conditions for the Xpress technology.
• Breast, placenta, lymph nodes, and other fatty tis-
sues may require longer immersion times in PPS (60
minutes).
• PPS to be changed every 3 hours.
• For Breast tissues requiring IHC testing, time in
10% NDB must be a minimum of 6 hours to satisfy
the ASCO guidelines.
Tissues in cassettes sectioned at final thickness of 2 mm should be immersed for 1 hour in 10% NBF.
Alternative step to address specific
Pathologists’ preferences-
Immersion in PPF for 30 minutes.
Alternative step for Formalin-Free
Tissue Processing-
Collect and fix tissues in Molecular Fixative,
then gross to final thickness and immerse in
Molecular Fixa
tive for 1 hour.
Place in Loading Station filled with PPS with
immersion time set for 15 minutes, and
reagent usage limit set at 3 hours.
Tissues in cassettes sectioned at final thickness of 3 mm should be immersed for 3 hours in 10% NBF.
Alternative step to address specific
Pathologists’ preferences-
Immersion in PPF for 30 minutes.
Alternative step for Formalin-Free
Tissue Processing-
Collect and fix tissues in Molecular Fixative,
then gross to final thickness and immerse in
Molecular Fixa
tive for 1 hour.
Place in Loading Station filled with PPS with
immersion time set for 30 minutes, and
reagent usage limit set at 3 hours.
Immersion in PPS for 30 minutes. (offline)
Immersion in PPS for 30 minutes. (offline)

Sakura Tissue-Tek Xpress x120 Specifications

General

Brand	Sakura
Model	Tissue-Tek Xpress x120
Category	Computer Hardware
Language	English