20 Operating Instructions Stainless Steel In-Line Filter Holder Type 16274
Operation
6 Operation
NOTICE
Damage to the device from improper use!
The coating of PTFE-coated device components may come away from the
base material if the device is operated with superheated steam or highly
diffusive substances, or if device surfaces are damaged.
t Do not operate the device with superheated steam or highly diffusive
substances.
t Avoid damage to the device surfaces.
6.1 Performing an Integrity Test
Ensure the integrity of the device before and after each sterile filtration
using the bubble-point-test.
Material: 1 silicone tube approx. 30 cm long
Pressure tubes
Requirement
The device is ready for use.
Procedure
t With a pressure tube, connect the inlet of the device to a pressure
source with a pressure gage.
t Insert the silicone tube on the valve on the lower part of the device.
t Immerse the other end of the silicone tube approx. 1 cm deep in water or
alcohol.
t Make sure that the valve is closed.
t Introduce a small amount of water or liquid to be filtered into the filler
opening.
t Seal the filler opening with the filler opening cap.
t To wet the membrane filter completely: Filter the liquid that was
introduced.
t If possible, seal the outlet of the device, e.g. with a stopcock:
t Seal the outlet of the device.
t Open the valve on the lower part of the device.
t If it is not possible to seal the outlet of the device:
t Make sure that the valve on the lower part of the device is closed.
t Push the end of the silicone tube not immersed in water or alcohol
onto the outlet of the device.
t Pressurize the device until 70% — 80% of the expected minimum bubble
point of the membrane filter is reached.
t Keep the pressure constant for 2 – 5 minutes.
t Then increase the pressure by approx. 0.1 bar every 5 seconds while
watching the end of the silicone tube that is immersed in water or
alcohol.
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