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INTRODUCTION
Note: A CO
2
gas regulator must be purchased separately from the incubator.
CO
2
GAS SUPPLY
Supply Required
The incubator must be connected to a carbon dioxide gas supply system in order to
establish and maintain a CO
2
-enriched incubation chamber atmosphere. The supply
can be a building CO
2
gas system or one or two supply cylinders (tanks).
Supply Quality
Use medical or food grade CO
2
. Use of industrial CO
2
risks introducing contaminants into the chamber,
may damage the incubator, and is not covered by the manufacturing defect warranty.
Supply Source and Pressure
The incubator requires 15 – 20 psi of CO
2
gas pressure at the incubator intake port (labeled CO
2
to
Chamber).
Dual-Stage Regulator
If connecting to a supply cylinder, always use a dual-stage CO
2
pressure regulator. During normal
operations, the incubator uses small quantities of CO
2
to maintain the chamber gas concentration.
Under these conditions, precise regulation of the gas input flow is vital for the incubator
performance. Some single-stage regulators have two gauges. Make certain your regulator is a
dual-stage regulator.
Regulator Not Included
See page 60 for ordering information.
Dual Cylinder Connection
The incubator can be connected simultaneously to two CO
2
supply cylinders. The unit
automatically switches drawing from one cylinder to the other when the first cylinder
runs out of CO
2
. Two gas regulators are required for this configuration.
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