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MAINTENANCE
CALIBRATE THE CO
2
DISPLAY
Note: This procedure requires a gas reference device. Please see the Reference Sensor Devices
entry on page 10 for the device requirements.
Gas calibrations are performed to match the incubator CO
2
display to the actual CO
2
concentration
inside the incubation chamber. The actual gas concentration is supplied by a calibrated reference
device. Calibrations compensate for long-term drifts in the incubator microprocessor controller as
well as those caused by the natural material evolution of the infrared sensor in the heated
recirculation duct space. Calibrate as often as required by your laboratory or production protocol, or
regulatory compliance schedule. Always calibrate to the standards and use the calibration setup
required by your industry requirements or laboratory protocol.
Humidity
Because humidity impacts CO
2
concentration through its influence on temperature stability and
uniformity, the CO
2
display should be calibrated with the chamber humidified.
A suggested calibration setup
1. Connect the reference device sample
tubing to CO
2
Sample Port located on the
top, right side of the incubator.
2. The incubation chamber door must be
closed and latched. Failure to do so will
prevent an accurate calibration.
3. CO
2
calibrations must be performed
with the chamber fully heated and
stable at your application temperature
setpoint. Temperature helps drive gas
diffusion in the chamber.
4. The incubator must be supplied with
CO
2
during the entire calibration
procedure.
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