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MAINTENANCE
5. The CO
2
concentration in the incubation chamber must be stable in order to perform an accurate
calibration.
• The unit must be thermally stable, having operated at your application temperature for at
least 12 hours prior to starting the gas calibration.
• After turning on a flow of CO
2
to the chamber and setting the CO
2
setpoint, allow the
incubator to operate undisturbed with the chamber door shut for at least 2 hours.
• To be considered stabilized, the incubator chamber must operate at your calibration CO
2
concentration for at least 30 minutes with no fluctuations of ±0.1% or greater.
Continued next page
A Suggested CO
2
Calibration
1
Once the incubator CO
2
concentration has stabilized, compare the
reference device and the CO
2
display readings.
• If the readings are the same, or the difference
between the two falls within the acceptable range of
your protocol, the display is accurately showing the
incubation chamber CO
2
concentration. The CO
2
Calibration procedure is now complete.
-OR-
• If the difference falls outside of your protocol range,
advance to Step 2.
Reference Device
Set CO
2
%
2
A display calibration adjustment must be entered to match the
display to the reference device. See next step.
Reference Device
X
(Minimum)
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