CyFlow
™
Space | Operating Manual | March 2021
15
Now the measurement (acquisition) starts automatically – the operating software indicates
Prerun, Stabilize, Run and Count phases. Instrument status is also highlighted by LEDs
on the front side of the CyFlow™ Space.
In the Prerun phase, cells are quickly transported into the flow cuvette.
During the Stabilize phase, sample flow stabilizes and the flow reaches the final set
sample injection rate.
In the Run phase, cells are analysed and data are acquired.
In the Count phase, cells are counted for a given volume. Do not change instrument
settings in the count phase! Do not clear data!
After the count phase, the acquisition finishes automatically. To finish the
acquisition before, click the END button, or simply remove the sample tube
from the sample port. In any case the instrument initiates now the
REPOSITION phase to prepare the instrument for the next analysing cycle.
or
A Biosafety cleaning cycle is started.
The system status changes to Clean and then Ready.
The system is ready for the next sample.
NOTICE
When removing a tube from the sample port, the BioSafety Cleaning
system is activated to prevent dropping and contamination by hazardous
material and to clean the sample tube. During Cleaning and Reposition
phases FloMax
®
does not accept any actions. Instrument operation can
only be continued after finalization of both phases.
To restart an acquisition without removing the sample tube, click onto start.
or
3. To save results, click on the save file icon.