SYSMEX CyFlow Space User Manual

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Operatin
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Manual
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March 2021
Cleaning of the flow cuvette
For cleaning the flow channel use Cleaning Solution (REF 04-4009_R) filled into a
standard sample tube and connect to the sample port 2-3 times. Then perform additional
cleaning cycles with destilled water filled into a fresh sample tube.
Never open the instrument to clean the flow cuvette mechanically e.g. by wires or tools for
cleaning or declogging the flow cuvette. Please contact your local Sysmex representative.
Removing Air Bubbles from Flow Cuvette
If you notice insufficient peak resolution during sample run remove your sample and
activate CLEAN several times. This way the flow cuvette is back flushed with sheath fluid.
Restart the sample and observe peak resolution again. If the results are still insufficient, run
Calibration Beads (e.g. Calibration Beads 3 µm, ready to use (REF 05-4018)). Compare
the result with the exemplary data files located in the FloMax/QC or FloMax/Calibration data
directory and observe the flow monitor (CMOS camera). A single bright spot should appear
in the center of the visible field. If this is not the case air bubbles or dirt inside the flow cell
may cause the disturbance. As first easy approach to release bubbles during system run
attach a tube with Cleaning Solution and pinch first the sheath and then the waste
tubing several times to remove air bubbles through the flow channel into the waste.
In case air bubbles are still persistent please apply the following procedure:
1. Select the instrument settings:
Sample pre-run = 15 sec
Stabilizing time = 5 sec
Automatic stop = 15 sec
2. Place an empty sample tube on sample port
3. Press START and wait till automatic stop
4. Place a tube with 1.5 ml Cleaning Solution on the sample port and repeat step 3.
5. Reset instrument settings to normal values
If analysis with Calibration Beads does not result in acceptable signal distributions an
incubation of the flow cell with Decontamination Solution is required. Run the system with
1.6 ml Decontamination Solution. During system RUN pinch the sheath fluid tube for 5
seconds. Stop the instrument while keeping the tube pinched by pressing STOP.
Incubate for at least 30 minutes. Re-start the system by pressing START and let it run to
the end. Decontamination Solution may incubate even over night! Wash the flow cuvette by
attaching a tube with 1.6 ml of Sheath Fluid and run it for at least 2 minutes.
In case your flow cytometer shows degraded performance even after thorough cleaning,
please contact your local Sysmex representative.

SYSMEX CyFlow Space Specifications

General

Brand	SYSMEX
Model	CyFlow Space
Category	Measuring Instruments
Language	English