4
C
Flow
™
Space
Operatin
Manual
March 2021
Table of figures
Figure 1: Cells are separated in a suspension and stained with fluorescent markers ..... 9
Figure 2: Light from the laser(s) excites cell-bound fluorophores and is scatted by the
cells .................................................................................................................. 9
Figure 3: Fluorescence signals are displayed and analysed in histogram and/or dot plot
diagrams ......................................................................................................... 10
Figure 4: True Volumetric Absolute Counting by the Electrode Principle ...................... 18
Figure 5: Exemplary demonstration of the optical bench of a CyFlow™ Space with 2
lasers and 8 parameters (left) and 3 lasers and 8 parameters (right). Other
laser light sources and parameter allocations are possible. D = detector (photo
multiplier) ........................................................................................................ 22
Figure 6: Optical geometry at the laser interrogation (schematics) ............................... 23
Figure 7: Multiparameter instrument settings box ......................................................... 24
Figure 8: The Parameter Setup Dialog Box ................................................................... 24
Figure 9: Sample Transporting System of the CyFlow™ Space. .................................. 28
Figure 10: Recommended placement of CyFlow™ Space components ......................... 32
Figure 11: Instrument Setup and periphery (CyFlow™ Space shown from the rear) ...... 33
Figure 12: Computer connection on the CyFlow™ Space rear ....................................... 34
Figure 13: Power connection and light source switches on the left side of the CyFlow™
Space. ............................................................................................................ 35
Figure 14: Tube connections for sheath and waste containers and cable connections for
the fluid level sensors on the right side of the CyFlow™ Space. .................... 36
Figure 15: Flow cuvette with sample, sheath fluid and waste connections. .................... 37
Figure 16: Warning laser radiation .................................................................................. 40
Figure 17: Additional explanation .................................................................................... 40
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