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CyFlow Space
SYSMEX CyFlow Space User Manual
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Table of Contents
Default Chapter
2
Table of Contents
2
1 Identification
5
Product Information
5
Manufacturer
5
2 Introduction
6
What Is the Cyflow™ Space
6
What Are the Applications for Which the Cyflow™ Space Can be Used
6
What Topics Are Covered by this Manual
6
What Other Manuals Are Available
6
What Should I Know before Operating the Cyflow™ Space
6
What Standards Were Applied for CE Compliance
7
What Typographical Conventions Are Used in this Manual
7
Symbols in this Document
7
Safety Information
7
3 Typical Steps of Particle Analysis
9
Preparation and Staining
9
Flow Cytometry Analysis
9
Real-Time Data Processing and Results
9
Figure 1: Cells Are Separated in a Suspension and Stained with Fluorescent Markers
9
Figure 2: Light from the Laser(S) Excites Cell-Bound Fluorophores and Is Scatted by the Cells
9
Absolute Cell Counting
10
Figure 3: Fluorescence Signals Are Displayed and Analysed in Histogram And/Or Dot Plot Diagrams
10
4 Safety
11
Laser Radiation Hazards
11
Electrical Hazards
11
Alterations to the Device
11
5 Operating Basics
12
Switching on the Cyflow™ Space
12
Check SHEATH and WASTE Bottle
12
Switch on the Instrument
12
Control of the 488 Nm Laser Output Power (for 200 Mw High Power Laser Only, Model Coherent Sapphire)
12
Switch on Peripheral Devices
12
Switch on Computer
12
Start Instrument Operating Software
13
Priming the Instrument (Initial Cleaning)
13
Multi Laser Measurements
13
Starting a Measurement
14
Switching off the Cyflow™ Space
16
6 True Volumetric Absolute Counting - Overview
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Non-Volumetric Flow Cytometric Techniques for Absolute Counting
17
True Volumetric Absolute Counting
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Precision and Reproducibility
18
Benefits of True Volumetric Absolute Counting
18
Figure 4: True Volumetric Absolute Counting by the Electrode Principle
18
Performing True Volumetric Absolute Counting
19
7 Optical Standard Setup - Parameters
21
Laser Instrument
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Laser Instrument (High Power Laser 488 Nm)
21
Other Parameter Allocations Possible
22
Figure 5: Exemplary Demonstration of the Optical Bench of a Cyflow™ Space with
22
8 Optical Standard Setup - Flow Geometry
23
Figure 6: Optical Geometry at the Laser Interrogation (Schematics)
23
9 Instrument Settings
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The Parameter Setup Dialog Box
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Figure 7: Multiparameter Instrument Settings Box
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Figure 8: the Parameter Setup Dialog Box
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Trigger
25
Gain
26
Sample Speed
27
Figure 9: Sample Transporting System of the Cyflow™ Space
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Threshold: Lower Level (L-L)
29
10 Disposal
30
Disposal of Device
30
Disposal of Components
30
Waste Disposal
30
Sheath Fluid Disposal
30
11 Appendix
32
Installation and Uninstallation
32
Installation Requirements
32
Figure 10: Recommended Placement of Cyflow™ Space Components
32
Figure 11: Instrument Setup and Periphery (Cyflow™ Space Shown from the Rear)
33
Instrument Setup - Step by Step
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Figure 12: Computer Connection on the Cyflow™ Space Rear
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Figure 13: Power Connection and Light Source Switches on the Left Side of the Cyflow™ Space
35
Figure 14: Tube Connections for Sheath and Waste Containers and Cable Connections for the Fluid Level Sensors on the Right Side of the Cyflow™ Space
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The Flow Cuvette
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Figure 15: Flow Cuvette with Sample, Sheath Fluid and Waste Connections
37
Maintenance and Service
39
Maintenance
39
Service
39
Transport and Storage
39
Disposal
39
Laser Safety
40
Figure 16: Warning Laser Radiation
40
Figure 17: Additional Explanation
40
12 Technical Data
41
System
41
Optics
42
Fluidics
43
Electronics and Computer
43
Software
44
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SYSMEX CyFlow Space Specifications
General
Brand
SYSMEX
Model
CyFlow Space
Category
Measuring Instruments
Language
English
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