8 D01-060en202008 Manual nanoFlu
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Introduction // nanoFlu
2.3.1 Fluorescence
Fluorescence is the spontaneous emission of light directly after a material has been excited. The emitted light is
generally lower in energy (larger wavelength) than the previously absorbed light (shorter wavelength).
Photons are absorbed and electrons of the molecule are lifted into an energetically higher orbital, i.e. excited. If
they fall back to their original level, the released energy produces heat and photons (uorescent light).
Double-bond electrons are excited more easily, because the p-electrons of the double bond are distributed over
both atoms and are therefore not so strongly bound. Molecules with a conjugated double bond are particularly
suitable for uorescence; the electrons are distributed over several atoms and are therefore very easy to excite.
2.3.2 Parameters
Depending on the parameters, nanoFlu uses dierent LEDs for long-term stable measurements of uorescence
values. The following parameters (see table) can be measured or derived with nanoFlu.
Parameter Excitation wavelength Detection wavelength
Chl-a 470 682
blue 620 655
CDOM 360 460
2.4 Browser
nanoFlu is equipped with a web interface, which can be used to congure the sensor. To access the web inter-
face, you will need the G2 interface box and an Ethernet-capable device with a web browser, e.g. a notebook.
Open one of the following URLs (depending on the network structure) in your web browser:
http://nanoFlu/ or
http:// nanoFlu _D2XX/ (D2XX is the serial number) or
http://192.168.77.1/
When connected to an Internet-capable device, automatic measurements will be stopped. As
soon as the sensor is disconnected from your device, the measurements will continue at the
set interval if the timer is activated for automatic measurements.
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