Turner Designs C3 - Raw Fluorescence Calibration - Blank Subtracted

C3 Submersible Fluorometer
998-2300 Rev. H Page 16
The temperature compensation coefficients for each fluorophore are
listed in the table below.
Fluorophore Coefficient
Rhodamine 0.026 / °C Exponential
Chlorophyll a 0.014 / °C Linear
Step 5: Save Calibration
♦ After calibration is complete, select “Save Calibration”.
♦ Data are now displayed as concentration estimates.
3.3.2 Raw Fluorescence Calibration – Blank Subtracted
Following steps 1-5 below will ensure values are reported as Blank
subtracted Relative Fluorescence Units (RFUB). See Appendix B for
recommended practices.
Step 1: Blanking
♦ Place the C3 Submersible Fluorometer in a blank solution.
Note: A blank solution is a solution without the fluorophore of
interest (i.e. de-ionized water, artificial water, or filtered water).
Note: Ensure the optical head is free of air bubbles.
♦ Wait until “Current RFU” readings have stabilized then select the “Set
Blank” button to set the blank value.
♦ Wait for the blank value to be measured and set for all 3 gains.
Step 2: Selecting Units
♦ Select RFUB from the units’ dropdown menu.
Note: Do not enter a value in the standard value box.
Step 3: Skip and go to Step 4
Step 4: Optional Temperature Compensation – Follow same steps
as the Direct Concentration Calibration procedure in Section 3.3.1.
Step 5: Save Calibration
♦ After calibration is complete select “Save Calibration”.
♦ Data are now displayed as blank corrected relative fluorescence
units.