998-2300 Rev. H Page 25
Appendix B: C3 Submersible Fluorometer Recommended Practices
Identification
Sensors are labeled 1, 2 or 3 adjacent to the optics on the face of the instrument.
Each channel is factory set in the C3 Windows Software.
Note: The channel labels can be modified in the C-Soft software.
Recommended Measurement Practices
The following steps will improve the accuracy and repeatability of your
measurements, especially at low concentration levels:
1. Use a dark plastic or glass container for your standards and/or samples. (Note:
Some plastics may fluoresce and might interfere with measurements.)
2. If using a glass beaker, place it on a non-reflective surface, preferably black.
3. Ensure that the sensor is >3 inches above the bottom of the container.
4. Ensure that the sensor is in the center of the container, and has >2 inches
clearance between the sensor and the inside surface of the container.
5. If using a glass beaker, check if the optical surface of the sensor is free of air
bubbles. Remove air bubbles if any are observed. If using a dark plastic
container, run the wiper or your finger over the optical sensors to ensure the
sensor heads are free of air bubbles.
>3
inches
Dark/Black
Non-Reflective
Surface
>2 inches all
round
Calibrated
Sensor
No Air Bubbles
On Optical
Surface
Glass Beaker
>3
inches
Dark/Black
Non-Reflective
Surface
>2 inches all
round
>2 inches all
round
Calibrated
Sensor
No Air Bubbles
On Optical
Surface
Glass Beaker
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