C3 Submersible Fluorometer
998-2300 Rev. H Page 15
3.3 Calibration Procedure
3.3.1 Direct Concentration Calibration
Following steps 1-5 below will ensure values are reported as blank
subtracted Concentrations in units of choice. See Appendix B for
recommended practices.
Step 1: Blanking
♦ Place the C3 Submersible Fluorometer in a blank solution and
ensure the optical head is free from air bubbles.
Note: A blank solution is a solution without the fluorophore of
interest (i.e. de-ionized water, artificial water, or filtered water).
♦ Wait until “Current RFU” readings have stabilized then select the “Set
Blank” button to set the blank value.
♦ Wait for the blank value to be measured and set for all 3 gains.
Step 2: Standard Value and Units
♦ Place the C3 Submersible Fluorometer in a standard solution or use
a secondary solid standard (see Appendix D for more information on
solid standards).
Note: UV and Turbidity sensors cannot be used with solid
standards.
♦ Enter the known concentration value of the standard solution or solid
standard in the “Standard Value” box. If the concentration is not
known, enter an arbitrary value.
♦ Enter manually or select desired units from the dropdown menu.
Note: Do not choose or enter “RFUB” if Direct Concentration
Mode is preferred.
Step 3: Set Standard
♦ When readings in “Current RFU” box have stabilized, select the “Set
Standard” button.
Step 4: Optional Temperature Compensation
Temperature compensation is optional; it enables the C3 to compensate
for changes in fluorescence due to varying temperatures as detected by
the C3’s temperature sensor. Temperature compensation is available for
Chlorophyll a and Rhodamine sensors only. Temperature is displayed in
degree Celsius.
♦ Click on the “Temperature Compensation” box.
♦ Select either Chlorophyll a or Rhodamine from the dropdown menu.
♦ Data will now be corrected for temperature fluctuations.
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