C3 Submersible Fluorometer
998-2300 Rev. H Page 14
Calibration Screen
Users can calibrate sensors in either the direct concentration mode, raw
fluorescence mode, or blank subtracted raw fluorescence mode.
Direct Concentration Mode
Values reported in the Direct Concentration Mode are scaled to a
predetermined standard value and blank subtracted. The equation used
to calculate concentrations after calibrating to Direct Concentration Mode
is:
Raw Fluorescence Blank Subtracted Mode
Values in the Raw Fluorescence Blank Subtracted Mode are noted as
RFUB. It is important to note that these values are blank subtracted but
are not scaled to a standard; they are relative values.
Raw Fluorescence Mode
Values in the Raw Fluorescence Mode are referred to as “Relative
Fluorescence Units” (RFU). It is important to note that these values are
not blank subtracted or scaled to a standard; they are relative values.
Standard Value (units)
Standard RFU
x Sample RFU = Concentration (units)
Standard Value (units)
Standard RFU
x Sample RFU = Concentration (units)
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