Table B–3: UV cutoff wavelengths for common chromatographic solvents
(continued)
Solvent UV cutoff (nm)
Diethyl amine 275
Ethanol 210
Isopropanol 205
Isopropyl ether 220
Methanol 205
n-Propanol 210
Tetrahydrofuran (THF) 230
B.2.4.2 Mixed mobile phases
The following table provides approximate wavelength cutoffs for some other
solvents, buffers, detergents, and mobile phases. The solvent concentrations
represented are those most commonly used. If you want to use a different
concentration, you can determine approximate absorbance using Beer’s law, because
absorbance is proportional to concentration.
Note: λ cutoff is the wavelength at which the absorbance of the solvent equals 1
AU.
Table B–4: Wavelength cutoffs for different mobile phases
Mobile phase UV cutoff
(nm)
Acetic acid, 1% 230
Ammonium acetate, 10 mM 205
Ammonium bicarbonate, 10 mM 190
Polyoxyethylene (35) lauryl ether (BRIJ 35), 0.1% 190
3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate)
(CHAPS) 0.1%
215
Diammonium phosphate, 50 mM 205
(Ethylenedinitrilo) tetraacetic acid disodium salt (disodium EDTA),
1 mM
190
4-(2-hydroxethyl)-1-piperazineet hanesulfonic acid (HEPES), 10
mM, pH 7.6
225
Hydrochloric acid, 0.1% 190
Morpholinoethanesulfonic acid (MES), 10 mM, pH 6.0 215
June 12, 2015, 715004747 Rev. A
Page 75
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