2 Performance optimization ...........................................................20
2.1 General guidelines ........................................................................................20
2.2 Carryover....................................................................................................21
2.3 Reproducibility ............................................................................................21
2.4 Cycle time (between injections)......................................................................21
2.5 Preventing leaks...........................................................................................22
2.5.1 Installation recommendations for fittings ................................................22
2.6 Developing methods .....................................................................................30
2.7 Measuring dwell volume ................................................................................30
2.8 Transferring methods....................................................................................31
2.9 Matching sample diluents ..............................................................................31
3 System preparation .....................................................................32
3.1 Powering-on the system ................................................................................32
3.2 Monitoring module LEDs ................................................................................33
3.2.1 Power LED..........................................................................................33
3.2.2 Status LEDs........................................................................................33
3.3 Monitoring from control panels .......................................................................35
3.3.1 SM FTN-R control panel........................................................................35
3.3.2 QSM-R control panel ............................................................................37
3.3.3 2489 UV-Vis control panel ....................................................................38
3.3.4 2998 PDA control panel ........................................................................39
3.3.5 2424 ELS control panel ........................................................................40
3.3.6 2475 FLR control panel.........................................................................41
3.3.7 2414 RI control panel...........................................................................43
3.4 Opening the console .....................................................................................44
3.4.1 Opening the console from Empower software ..........................................44
3.4.2 Opening the console from MassLynx software..........................................44
3.5 Priming the system.......................................................................................44
June 12, 2015, 715004747 Rev. A
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