38
Advanced
™
Osmometer Model 3250 User’s Guide
Sample test procedure
1. Remove the sample tube from the freezing chamber (see Figure 3,
Item 8).
2. Gently wipe the probe, stir/freeze wire, mandrel and the top of the
freezing chamber (in that order) with a soft, lint-free, non-ionic
paper tissue dampened with distilled water to remove anything that
might contaminate the sample to be tested. Be careful not to bend
the probe or stir/freeze wire.
3. Select a reference solution or NaCl standard with a freezing point
close to that expected for your unknown (Advanced
™
Clinitrol
™
290
Reference Solution is recommended for serum). Swirl and carefully
open an ampule of the reference solution (or NaCl standard)
selected.
4. If the expected freezing point is in the 1500-4000 mOsm range, the
high range should be selected. If the freezing point is not within the
range selected, an error message will be displayed, requesting the
range to be changed (see Setup Menu, later in this chapter).
5. Select a clean sample tube (see Repeatability Tips).
6. Pipette or measure a sample of the selected reference solution or
NaCl standard into the clean sample tube and place the tube in the
freezing chamber.
7. Press the START key.
I.D. #: If I.D. # has been enabled via the setup menu (see
3. Dis/Enable I.D. #), the instrument operator will be
prompted for a sample or user identification number of up
to 13 digits at this time from either the keypad or optional
barcode scanner.
a. At the I.D. # prompt, the present setting may be retained by
pressing ENTER.
b. The present setting may be erased entirely by pressing CLEAR or
erased one digit at a time from the right by repeatedly pressing <.