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Advanced Instruments Osmometer 3250 - Service Manual

Advanced Instruments Osmometer 3250
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3255SM Rev. 25
The Advanced™ Osmometer 3250
The Advanced™ Cryoscope 4250
Service Manual

Table of Contents

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Summary

Advanced Osmometer 3250; Cryoscope 4250 Service Manual

Safe Use

Symbol Conventions

Explains symbols used in the manual for safety and operation.

General Cautions and Requirements

General Cautions

Provides essential safety guidelines for operating the instrument.

Service and Maintenance Cautions

Warns about hazards during service and maintenance procedures.

Hazardous Material Cautions

Advises on handling hazardous materials and biohazards.

FCC Requirements

Details FCC rules for device operation and compliance.

Supplies, Parts, and Accessories

3250 Supplies, Parts, and Accessories

Lists consumables, spare parts, and accessories for the Model 3250.

3250 Controls and Calibration Standards

Lists calibration standards and control solutions for the Model 3250.

4250 Supplies, Parts, and Accessories

Lists consumables, spare parts, and accessories for the Model 4250.

4250 Reference Solutions and Calibration Standards

Lists reference solutions and calibration standards for the Model 4250.

Overview of Instrument

Product Description

Describes the function and purpose of the osmometers and cryoscopes.

The Service Manual

Outlines the content and purpose of the service manual.

Service Assistance

Provides contact information for technical support and service.

Instrument Components

3250;4250 Components Diagram

Identifies main internal components of the instruments with diagrams.

Calibration Procedures

Calibration Overview

Explains calibration ranges and requirements for Model 3250 and 4250.

Calibration Procedure

Step-by-step guide for performing instrument calibration.

Circuit Description

Power Supply (Serial Suffix A - C)

Describes the power supply circuit for specific serial suffixes.

Power Supply (Serial Suffix D and Higher)

Describes the power supply circuit for later serial suffixes.

Front End Components

Explains the function of the sample thermistor and thermoelectrics.

Control Board Set Description

Details the control board set configuration.

Processor Board Description

Describes the processor board, memory map, and real-time clock.

PCB605 Application Board

Details the application PCB and its functions, including ADC, I/O ports, and connectors.

Other Circuit Components

Explains drive circuitry, thermoelectric cooler control, and head motor control.

Head Sensor Board Description

Describes the head sensor board and its optical sensors.

Keypad Transition Board Description

Describes the board interfacing the keypad and display.

Barcode Port Connections

Details the barcode port specifications and programming requirements.

Internal Printer Descriptions

Describes the internal printer assembly for different serial suffixes.

Cleaning and Maintenance

Flushing the System

Procedure for flushing the cooling assembly with a bleach solution.

Draining the System

Steps to drain the cooling system fluid.

Removing Covers

Instructions for safely removing the instrument covers.

Osmometer Well Cleaning

Detailed steps for cleaning the osmometer's sample well.

Cryoscope Well Cleaning

Detailed steps for cleaning the cryoscope's sample well.

Printer Cleaning and Lubrication

Procedure for cleaning and lubricating the printer mechanism.

Probe and Stir;Freeze Wire Alignment

Importance of alignment and reference to the alignment tool instructions.

Troubleshooting Guide

Troubleshooting Introduction

Overview of troubleshooting, error messages, and common causes.

Freeze Buzz Amplitude Check

Procedure to check and reset the freeze amplitude setting.

Stir Driver Noise (Serial Suffix A - C)

Identifies and addresses issues with stir driver noise in specific models.

Head Sensor Adjustment

Steps to adjust the head position sensors for proper operation.

Head Up;Down Test

Verifies the functionality of the head motor and sensors.

Power On Diagnostics

Explains the instrument's self-check upon power-up.

A;D Tests

Details analog-to-digital tests for probe and cooling assembly diagnostics.

Sample Bin Setting

Procedure for setting or verifying the sample bin number.

Block Bin Setting

Procedure for setting or verifying the block probe bin number.

Stir Amplitude Adjustment

How to check and adjust the stir amplitude setting.

Display;Printer Test

Tests the display and printer output for proper function.

Beeper Test

Tests the internal beeper functionality.

Keypad Test

Verifies the functionality of each key on the keypad.

Barcode Test

Tests the instrument's ability to read barcodes.

Reset Serial Number

Procedure to reset and enter the instrument's serial number.

Instrument Software Updates

Information on updating instrument firmware via PC.

Troubleshooting Table Entries

Comprehensive table of symptoms, explanations, and suggested actions.

Performing FLASH Firmware Update

Method without opening cover

Steps for updating firmware without disassembling the instrument.

Method with cover removed

Steps for updating firmware with the instrument cover removed.

Option Switch Settings

Replacement Instructions

Mandrel Replacement

Step-by-step guide for replacing the mandrel.

Clapper Replacement

Instructions for replacing the clapper assembly.

Control Board Set Replacement

Guide for replacing the main control board set.

Application Board Replacement

Instructions for replacing only the application board.

Processor Board Replacement

Instructions for replacing only the processor board.

Cooling Assembly Replacement

Instructions for replacing the cooling assembly.

Cooling Fan Replacement

Steps for replacing the cooling fan.

Display Board Replacement

Instructions for replacing the display board.

Fuse Replacement

Procedure for replacing instrument fuses.

Driver Board Replacement

Instructions for replacing the driver board (Serial Suffix D and higher).

Head Up;Down Relay Replacement

Steps for replacing head up/down relays (Serial Suffix A-C).

Heat Transfer Fluid Pump Assembly Replacement

Instructions for replacing the heat transfer fluid pump assembly.

Fluid Filter Replacement

Instructions for replacing the fluid filter.

One-way Check Valve Replacement

Steps for replacing one-way check valves.

Keypad Replacement

Detailed procedure for replacing the keypad assembly.

Load Resistor Replacement

Instructions for replacing the load resistor.

Motor Assembly Replacement

Steps for replacing the motor assembly.

NVRAM Battery Replacement

Procedure for replacing the NVRAM battery.

Power Supply Assembly Replacement

Instructions for replacing the power supply assembly.

Printer Assembly Replacement (325400 R)

Steps for replacing the printer assembly (Serial Suffix A-C).

Printer Assembly Replacement (325420 R)

Instructions for replacing the printer assembly (Serial Suffix D and higher).

Replacing the Printer Mechanism

Steps for replacing the printer mechanism.

Probe Alignment Instructions

Guide for aligning the probe, mandrel, and stir/freeze wire.

Sample Probe Replacement

Procedure for replacing the sample probe.

Stir;Freeze Coil Replacement

Instructions for replacing the stir/freeze coil.

Stir;Freeze Wire Replacement

Steps for replacing the stir/freeze wire.

PCB520 Transistor Replacement

Guide for replacing a specific transistor (for older models).

PCB522 Transistor Replacement

Guide for replacing a specific transistor (for older models).

Tube Ejector Spring Replacement

Instructions for replacing the tube ejector spring.

Yoke Replacement

Steps for replacing the yoke assembly.

Upgrade Kit - 3250;4250 Suffix D to E

Instructions for upgrading the instrument to Serial Suffix E.

Main Harness Replacement

Instructions for replacing the main harness.

Schematics and Drawings

PCB402 Assembly Drawing Rev0

Assembly drawing for PCB402.

PCB402 Assembly Drawing Rev1

Assembly drawing revision for PCB402.

PCB402 Printer Interface Board Schematic Rev0

Schematic for the PCB402 printer interface board.

PCB402 Printer Interface PCB402 Schematic Rev1

Schematic revision for the PCB402 printer interface board.

PCB523 Assembly Drawing Rev0

Assembly drawing for PCB523.

PCB523 Assembly Drawing Rev4

Assembly drawing revision for PCB523.

PCB523 Driver Board Schematic Rev0

Schematic for the PCB523 driver board.

PCB523 Schematic Rev2

Schematic revision for PCB523.

PCB605 Assembly Drawing Rev 11

Assembly drawing for PCB605.

PCB605 Application Board Schematic Rev2

Schematic for the PCB605 application board.

PCB605 Schematic Rev5

Schematic revision for PCB605.

PCB620 Assembly Drawing

Assembly drawing for PCB620.

PCB620 Processor Board Schematic Rev4

Schematic for the PCB620 processor board.

210102 Assembly Drawing Rev1

Assembly drawing for 210102.

210102 Schematic Rev1

Schematic for 210102.

3 D3380 Assembly Drawing Rev5

Assembly drawing for 3D3380.

3 D3380 Assembly Drawing Rev9

Assembly drawing revision for 3D3380.

3 D3380 Schematic Rev4

Schematic for 3D3380.

3 D3380 Schematic Rev5

Schematic revision for 3D3380.

325000 Interconnection Schematic Rev4

Interconnection schematic for specific serial suffixes.

325000 Interconnection Schematic Rev5

Interconnection schematic for specific serial suffixes.

325000 Interconnection Schematic Rev6 for Serial Suffix C

Interconnection schematic for specific serial suffixes.

325000 Interconnection Schematic Rev6 for Serial Suffix D

Interconnection schematic for specific serial suffixes.

325000 Interconnection Schematic Rev8 for Serial Suffix D

Interconnection schematic for specific serial suffixes.

325000 Interconnection Schematic Rev8 for Serial Suffix E

Interconnection schematic for specific serial suffixes.

325000 Interconnection Schematic Rev11 for Serial Suffix E

Interconnection schematic for specific serial suffixes.

Model 3250 Specifications

Serial Suffix A - C Specifications

Specifications for Model 3250, Serial Suffixes A-C.

Electrical Specifications

Details electrical specifications like voltage, fuses, and power.

Performance Under Reference Conditions

Specifies linearity and repeatability under reference conditions.

Performance Under Operating Conditions

Details performance variations based on operating conditions.

Operating Conditions

Lists operating temperature, humidity, and storage requirements.

Serial Suffix D and Higher Specifications

Specifications for Model 3250, Serial Suffixes D and higher.

Physical and Warranty Information

Provides dimensions, weight, warranty, and certification details.

3250 Design Changes by Revision

Details changes made per serial suffix revision for the Model 3250.

Model 4250 Specifications

Serial Suffix A - C Specifications

Specifications for Model 4250, Serial Suffixes A-C.

Electrical Specifications

Details electrical specifications like voltage, fuses, and power.

Performance Under Reference Conditions

Specifies linearity and repeatability under reference conditions.

Performance Under Operating Conditions

Details performance variations based on operating conditions.

Operating Conditions

Lists operating temperature, humidity, and storage requirements.

Serial Suffix D and Higher Specifications

Specifications for Model 4250, Serial Suffixes D and higher.

Physical and Warranty Information

Provides dimensions, weight, warranty, and certification details.

4250 Design Changes by Revision

Details changes made per serial suffix revision for the Model 4250.

Regulatory Notices

Product Compliance Statements

Statements regarding compliance with US, Canadian, and European regulations.

Symbol Conventions

Explains regulatory symbols related to product compliance and safety.

Regulatory Approval Type Descriptions

Lists regulatory approvals and their applicability based on serial suffix.

General Symbol Definitions

Defines various symbols used in the manual for controls, tests, and warnings.

Replacement Parts

3250 Common Parts List

Lists common replacement parts for the Model 3250.

4250 Common Parts List

Lists common replacement parts for the Model 4250.

Parts for Serial Suffix A-C

Lists parts specific to Serial Suffix A-C models.

Parts for Serial Suffix D and Higher

Lists parts specific to Serial Suffix D and higher models.

Product Disposal and Recycling

Disposal and Recycling Guidelines

Provides guidance on environmentally responsible product disposal.

Service Log

Service Log Table

A log to record maintenance and service history.

Advanced Instruments Osmometer 3250 Specifications

General IconGeneral
Model3250
Sample Volume20 µL
Accuracy±2 mOsm/kg H2O
Resolution1 mOsm/kg H2O
Measurement Time90 seconds
Power Requirements100-240 V, 50/60 Hz
Measurement Range0 to 4000 mOsm/kg H₂O
Operating Temperature15° to 30°C (59° to 86°F)
CalibrationAutomatic
Data OutputRS-232
DisplayLCD
Storage Temperature0°C to 50°C
TypeFreezing Point Depression Osmometer
Repeatability±2 mOsm/kg H2O

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