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Advanced
™
Osmometer Model 3250 User’s Guide
Note Results are displayed with the (-) negative temperature sign
removed, per industry practice. However, if testing a sample
with a near 0 mOsm value, it may be possible for a positive
temperature value to be displayed with the negative sign
preceding the mOsm value.
9. Remove the sample tube from the cooling well.
CAUTION The heat transfer fluid contains hazardous chemicals.
Consult the material safety data sheet (MSDS) and use
appropriate personal protective equipment.
CAUTION Heat transfer fluid can become contaminated with sample
material which may be considered a biohazard. Use
appropriate cautions during removal and disposal of sample
tubes after testing to prevent heat transfer fluid remaining
on the tube exterior from contacting personnel or the
instrument surface.
10. Gently wipe the probe, stir/freeze wire, mandrel and the top of the
freezing chamber (in that order) with a soft, clean and dry, lint-free,
single-use paper tissue after each test, to avoid contaminating the
next sample. Be careful not to bend the probe or stir/freeze wire.
11. Repeating steps 1-9, test two to four more aliquots of the same
reference solution or standard to check repeatability and accuracy
before running tests on unknown samples. Follow the techniques
recommended in Repeatability Tips. When checking calibration and
repeatability with standards or reference solutions, it is usually best
to average the readings from three to five aliquots of each sample
to avoid error.
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