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Gating
Gating is used to restrict the number of events that are evaluated by gating out (filtering)
events that do not have the fluorescence values set by a marker. For example, by gating
on a blue marker, you can exclude all events with low blue fluorescence (allowing you, for
example, to gate out dead cells, unbound dye and debris). Only events with blue
fluorescence values within the marker range are evaluated. Thereby, you can exclude any
dead cells, and evaluate only the living cells for another property.
The gating direction defines the reference histogram:
• Gating from blue to red uses the blue histogram to define the subset by a marker
(Apoptosis and Antibody Staining assays).
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