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The following image shows two histograms with a gating direction from blue to red (left
to right) of an apoptosis assay. The blue histogram shows calcein fluorescence, which
indicates living or dead cells (high fluorescence value means living cells). The red
histogram shows the subpopulation of living cells with annexin V fluorescence indicating
apoptosis (high fluorescence value means the cell is apoptotic). As a result you can see a
subset of living, apoptotic cells.
Number of
events
Fluorescence
value
Histogram
Marker
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