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Histogram Evaluation
The blue histogram is used for gating. High fluorescence in the blue histogram means
that the cells are living (if a life-indicating dye is used). Low fluorescence means the cells
are dead. If you use a nucleic acid dye, you cannot distinguish between living and dead
cells, you can only count all measured cells. See the following image as an example:
The values are displayed in the result table below the blue histogram:
Low fluorescence
value indicates dead
or non-healthy cells
High fluorescence
value indicates living
cells
All measured events
All events in relation to
the blue marker (here
calcein)
Living cells related to all measured cells (high calcein fluorescence)
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