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When using the calcein marker in the blue histogram for gating, only living cells are
considered for building the histogram of the red dye. High red fluorescence values
indicate living cells with bound antibodies, low red fluorescence values living cells
without bound antibodies. See the following example.
The values are displayed in the result table below the red histogram:
Low fluorescence values
indicate low binding of
labeled antibodies
High fluorescence
values indicate binding
of labeled antibodies
Percentage of all cells with
high red fluorescence
selected by the red marker
Percentage of the living cells
Amount of living cells with high red fluorescence in relation to the amount of living cells
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