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Agilent Technologies 2100 - Page 97

Agilent Technologies 2100
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Contents 97 Index
Two categories of anomalies were introduced, critical and non-critical. Anomalies in
regions interfering with the customer sample RNA are considered critical. The
corresponding gel lane is flagged red.
The baseline anomaly, for example, is detected for signals with fluctuating or steep
baseline. The ribosomal ratio anomaly detects unexpected ratios of the 28S fragment
area and the 18S fragment area. The unexpected sample type anomaly is detected for
samples which do not fit the standard total RNA profile.
Anomaly Description Critical?
Unexpected baseline signal Yes
Unexpected signal in pre-region No
Unexpected signal in 5S-region Yes
Unexpected signal in fast-region Yes
Unexpected signal in inter-region Yes
Unexpected signal in precursor-region No
Unexpected signal in post-region No
Unexpected ribosomal ratio Yes
Unexpected sample type Yes
Unexpected lower marker (compared to previous well) No

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