Exercise 1 – Develop an acquisition method
Task 1. Enter acquisition parameters and acquire data
10 Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
Steps Detailed Instructions Comments
3 Acquire data (optional).
• Set up a one-line worklist with
the method you just created.
• Name the data file
iiisulfamix01.d, where iii are
your initials.
• Designate a directory path to
hold your data files and method.
a If necessary, click View > Worklist to
display the Worklist window.
b Click Worklist > Worklist Run
Parameters. Verify that the
parameters are set properly. Click OK.
c Click Worklist > Add Multiple
Samples.
d Type iii
sulfamix01.d as the
data file name
e Select iiiMS2Scantest.m as the
method name.
f Click the Sample Position tab.
g Select the Autosampler, Well-plate or
Vial Tray.
h In the graphic, select a single position.
Click OK.
i In the Worklist window, mark the
check box to the left of the sample.
j Click the Start Worklist Run icon in
the main toolbar, the Run Worklist
icon in the Worklist toolbar or click the
Worklist > Run command.
• The Worklist window is tabbed with
the Method Editor window by
default. Click the Worklist tab to
show the Worklist window.
• The Number of samples is set to 1.
• You have just acquired a full scan
MS data file to see what ions are
being formed from the sample.
• This step is optional because you
can perform the next step with an
example data file that comes with
the program. If you prefer, you can
create your own data file as
described in this step.
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