Exercise 1 – Develop an acquisition method
Task 3. Find optimum fragmentor voltage for maximum response
18 Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
d If necessary, click Define
Chromatograms in the Chromatogram
section of the Method Explorer.
e To delete the BPC chromatogram, click
Delete in the Method Editor window.
f Select EIC for the Chromatogram
Definition Type,
g In the MS Chromatogram tab, make
sure MS Level is set to All and Scans
is set to All scan types.
h Clear the Do cycle sum check box.
i Type
271 as the m/z value.
j Click Add.
k Repeat steps i and j for the other
precursor ions,
279, 285 and
311.
l Click Method > Save As. The system
opens the Save As dialog box
m Save the method as iiiExercise 1.m.
n Click Save.
• The default Method Editor list
selection after installation is
Integrate (MS).
• You can also select Define
Chromatograms from the Method
Items list in the Method Editor
window.
• When you are defining
chromatograms, instead of deleting
the Defined chromatogram, you can
select EIC. Then, you enter the m/z
value and click the Change button.
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