Exercise 1 – Develop an acquisition method
Task 4. Determine product ion masses
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide 27
q Click General in Method Explorer, and
then click File Open Actions.
r Select Integrate and extract peak
spectra from the Available actions list
and click to add this to
Actions to be run.
Figure 5 General > File Open Actions tab
s To apply the changes to the current
method, iiiexercise1.m, click the Save
Method icon. You can also click
Method > Save.
4 Run the qualitative method on the
current data file.
• In the Method Editor toolbar, click the
Run button, . When the Assign
Actions to Run Opening A Data File
section is displayed, the Actions to be
run list is run.
• The program first extracts the
product ion chromatograms for
each precursor ion in the data file.
• Next, it finds the largest peak in the
total ion chromatograms, and
integrates and extracts peak
spectra from each integrated peak.
• See Figure 6 on page 28.
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