Exercise 4 – Optimize Acquisition parameters
Task 1. Use Optimizer to optimize acquisition parameters
62 Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
3 Select the precursor ions a Click the Precursor Ion Selection tab.
b Select the Positive ions +H adduct.
c Select the Charge state of 1.
d Set the search priority of the precursor
ions.
e (optional) To exclude certain masses
from consideration, click Exclude
masses at the bottom of the screen.
Enter the m/z Values to exclude
separated by commas and/or enter a
Minimum abundance value in counts.
• Mark the Use most abundant
precursor ion check box to use the
most abundant precursor ion.
• Clear the Use most abundant
precursor ion check box and use
the Up and Down arrow buttons to
set the search order (ions at the top
of the list are given more priority).
• You can also enter Neutral Losses
to exclude (for example H
2
O).
••
Steps Detailed Instructions Comments