Exercise 4 – Optimize Acquisition parameters
Task 1. Use Optimizer to optimize acquisition parameters
64 Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
5 Set up a compound list. The
formula for the four Sulfa Drugs
are:
• Sulfamethizole
C
9
H
10
O
2
N
4
S
2
• Sulfamethazine
C
12
H
14
O
2
N
4
S
• Sulfachloropyridazine
C
10
H
9
O
2
N
4
SCl
• Sulfadimethoxine
C
12
H
14
O
4
N
4
S
a Click the Compound Setup tab.
b Clear the Show results summary
check box above the table while you
set up the compound list.
c Right-click the table and select Add
Compound from the shortcut menu to
add a row to the end of the table.
d Enter
Sulfamethizole as the
Compound Name.
e Enter
Sulfa drugs as the group
name in the Groups column.
f Enter
C9H10O2N4S2 as the
Formula of the compound. The mass
is calculated.
g Enter the Sample Position for the new
compound.
h (optional) Enter an Optimization dwell
time value to set longer or shorter
cycle times.
i Repeat the steps above to add the
other three sulfa drugs to the table.
j Mark the Select columns for the
compounds (rows) to use for
optimization.
k Save the compound list to the
database or to the current project.
• Compounds are global to all
projects. Compound information
such as name, group, formula, and
mass in one project will be
reflected in the entire database.
• If no methods or ions are specified
here, then optimization for the
compound uses the methods from
the Optimizer Setup tab and
information from the Precursor Ion
Selection and Product Ion Selection
tabs to generate the ions.
• You can also enter the
monoisotopic mass in the Mass
column instead of the Formula.
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