APPENDIX - Examples
Agilent 7100 CE in Empower Environment 37
APPENDIX - Examples
Example: Single test run
For testing the functions of the 7100 CE and Empower 3 a test method can
be created. The equipment and method parameters can be summarized as
follows:
Capillary: Fused silica capillary 50 µm ID, 48.5 cm total (40 cm effective),
ext. Light path (G1600-60232)
20 mM Borate (from IQ-Kit: 5063-6514)
Home vials: 1 and 2 (filled with electrolyte solution)
Sample: 4-Hydroxyacetophenone solution 1 mmol/l (from IQ-Kit: 5063-6514)
NOTE
The sample has to be diluted 1:10 before the run.
Voltage: +30 kV
Temperature: 25°C
Stop Time: 4 min
Preconditioning: 300 s (from electrolyte vial 3 into waste vial)
50 mbar for 10 s (from injection vial to outlet vial)
Post-injection: 50 mbar for 5 s (from inlet vial to outlet vial)
DAD: Signal: 200 nm (BW 5 nm)
Reference: 360 nm (BW 100 nm)
Peakwidth: >0.025 min (10 Hz)
DAD Timetable: 0.5 min Balance
The run can be executed as single run (in this case save the instrument
method as well as the method set). If defined in the method, the online plot
shows the selected signals and the final result electropherogram can be
obtained in the Browse menu of Empower or via the projects.
NOTE
Ensure that the Run Time is the same as defined in the CE/DAD method.
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