Using the CE in Empower
Agilent 7100 CE in Empower Environment 25
Replenishment and Conditioning
Replenishment allows to automatically change the buffer of a vial either in
serial or parallel mode.
Preconditioning and Postconditioning offers conditioning of the capillary.
These settings are method settings and therefore expected each time the
method is running. If this is not wanted, make sure to generate methods
with and without replenishment and/or pre/postconditioning.
During multiple analyses the buffer must be refreshed after a certain
number of runs. Typically, the exchange of the buffer is done every 3 - 10
runs. Depending on the stability of the buffer, it is sometimes necessary to
refresh it before each run.
NOTE
Using the replenishment as a part of the method, the replenishment is carried out for
each run where this is part of the method.
If you want the refresh to be done less, ensure to create at least two methods.
Example:
Run 1 method A with replenishment
Run 2 method
Run 3 method
Run 4 method with replenishment
Run 5 method
Run 6 method
Run 7 method with replenishment
Run 8 method
Run 9 method
The replenishment system provides a quick way to change the buffer
automatically. The system removes the used buffer from the vials and
transfers it into the waste bottle. Then the vials are filled with fresh buffer
from the electrolyte reservoir.
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