Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual 63
Good Measurement Practices 4
General Considerations
✔ Blank measurement shows a flat baseline (Figure 9 and Figure 10 on
page 64 show a good and a poor baseline)
✔ Cell orientation of blank and sample measurements is the same
✔ Ideally the cell is not removed between the measurements, which means the
cell is filled/rinsed using a pipette or a flow cell is used
✔ Time between blank and sample measurement should be short
Figure 9 Example of a blank on water showing a good baseline
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