Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual 73
Using your UV-Visible Spectroscopy System 5
Measuring Caffeine Absorbance at 273 nm
6 Press the Blank button on the front of the spectrophotometer or click Blank
on the Instrument Panel to start the measurement.
7 Remember the orientation of the cell in the cell holder. Lift the level to
release the cell, remove it and flush it three times with about 1 ml of your
caffeine sample. Then fill the cell with about 3 ml of the caffeine sample.
Make sure that the cell windows are clean and reposition the cell in the
same orientation as for the reference measurement. Close the cell holder’s
level.
8 Press the Sample button on the front of your spectrophotometer, or click
Sample on the Instrument Panel to start the measurement.
9 The view shows you the spectrum of your caffeine sample with a vertical
line indicating your wavelength of interest. Below the spectrum is the
Sample/Result Table which shows the absorbance reading at 273 nm.
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