Agilent InfinityLab LC Series VWD User Manual 58
4 Preparing the Module
Setting up an Analysis
1 Open the purge valve of your pump (by turning it counterclockwise) and set
flow rate to 3–5mL/min.
2 Flush all tubes with at least 30 mL of solvent.
3 Set flow to required value of your application and close the purge valve.
Table 7 Choice of Priming Solvents for Different Purposes
Activity Solvent Comments
After an installation
When switching between reverse
phase and normal phase (both times)
Isopropanol
Isopropanol
Best solvent to flush air out of the sys-
tem
Best solvent to flush air out of the sys-
tem
After an installation Ethanol or Methanol Alternative to Isopropanol (second
choice) if no Isopropanol is available
To clean the system when using buf-
fers
After a solvent change
Bidistilled water
Bidistilled water
Best solvent to re-dissolve buffer crys-
tals
Best solvent to re-dissolve buffer crys-
tals
After the installation of normal phase
seals (P/N 0905-1420)
Hexane + 5% Isopropanol Good wetting properties
The pump should never be used for priming empty tubings (never let the pump
run dry). Use a syringe to draw enough solvent for completely filling the tubings
to the pump inlet before continuing to prime with the pump.
Pump for approximately 10minutes before starting your application.
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