Agilent InfinityLab LC Series VWD User Manual 59
4 Preparing the Module
Setting up an Analysis
Requirements and Conditions
What You Will Need
The table below lists the items you need to have for the set up of the analysis.
Some of these are optional (not required for the basic system).
Conditions
A single injection of the isocratic test standard is made under the conditions
given in Table 9 on page 59:
Table 8 What you will need
Agilent 1200 Infinity Series system Pump (plus degassing)
Autosampler
Detector, standard flow cell installed
Degasser (optional)
Column Compartment (optional)
Agilent CDS
System should be correctly set up for LAN com-
munication with the Agilent ChemStation
Column: Zorbax Eclipse XDB-C18, 4.6 x 150 mm, 5 µm
(993967-902) or an equivalent column
Standard: Agilent isocratic checkout sample (01080-68704)
Table 9 Conditions
Flow 1.5 mL/min
Stoptime 8 min
Solvent 100% (30% water/70% Acetonitrile)
Temperature Ambient
Wavelength sample 254 nm
Injection Volume 1 µL
Column Temperature (optional): 25 °C or ambient
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