Agilent InfinityLab LC Series VWD User Manual 73
5 Optimizing the Detector
Match the Flow Cell to the Column
Figure 21 Influence of Cell Path Length on Signal Height
Traditionally LC analysis with UV detectors is based on comparing
measurements with internal or external standards. To check photometric
accuracy of the detector, it is necessary to have more precise information on
path lengths of the flow cells.
The correct response is:
expected response * correction factor
Please find below the details of the flow cells:
Absorbance
Time (min)
Analysis of pesticide standard
10-mm optical path length
6-mm optical path length
Table 10 Correction factors for Agilent VWD flow cells
Part number Path length (actual) Correction factor
Standard flow cell 10 mm, 14 µL, 40 bar (G1314-60186) 10.15 ± 0.19 mm 10/10.15
Semi-micro flow cell 6 mm, 5 µL (G1314-60183) 6.10 ± 0.19 mm 6/6.10
Micro flow cell 3 mm, 2 µL, 120 bar (G1314-60187) 2.80 ± 0.19 mm 3/2.8
High pressure flow cell 10 mm, 14 µL, 400 bar (G1314-60182) 10.00 ± 0.19 mm 10/10
However you have to be aware that there is additional tolerance of gasket
thickness and its compression ratio which is supposed to be very small in
comparison with the machining tolerance.
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