Agilent InfinityLab LC Series Vialsamplers User Manual 141
4 Preparing the Module
Preparing the Module
Preparing the Module
For best performance of the module
• When using the module in a system with a vacuum degassing unit, shortly
degas your samples before using them in the module.
• Filter samples before use in a 1200 Infinity Series system. Use High pressure
filter kit (5067-4638) for inline filtering.
• When using buffer solutions, flush the system with water before switching it
off.
• Check the module plungers for scratches, grooves and dents when changing
the piston seal. Damaged plungers cause micro leaks and will decrease the
lifetime of the seal.
• Solvent Information - Observe recommendations on the use of solvents, see
“Solvent Information” on page 142.
• Priming and Purging the System - When the solvents have been exchanged or
the system has been turned off for a certain time (for example, overnight)
oxygen will re-diffuse into the solvent channel. Therefore priming and purging
of the system is required before starting an application.
Table 13 Choice of Priming Solvents for Different Purposes
Activity Solvent Comments
After an installation Isopropanol Best solvent to flush air out of
the system
When switching between
reverse phase and normal
phase (both times)
Isopropanol Best solvent to flush air out of
the system
After an installation Ethanol or methanol Alternative to isopropanol (sec-
ond choice) if no isopropanol is
available
To clean the system when
using buffers
Bidistilled water Best solvent to re-dissolve buf-
fer crystals
After a solvent change Bidistilled water Best solvent to re-dissolve buf-
fer crystals
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