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AmScope 330 Series - Focusing

AmScope 330 Series
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330
Series
Operation
11
6.
To adjust the illumination, slowly turn the dimmer knob on the right side of the body until the
desired intensity
of light is achieved. You may also adjust the iris on the light source itself by
rotating the ring around the base lens
or the iris on the condenser, so please make sure all are
adjusted to the correct positions.
Focusing
1.
Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4x
objective) to locate and focus on the specimen. As you move up in magnification you may need
to refocus the image a little each
time.
2.
When using the 100x objective, a drop of immersion oil should be placed between the cover slip
and the objective to minimize distortion caused by air. Always be sure to wipe the oil off of the
objective with either lens cleaning
paper or a nonabrasive, lint free cloth.
3.
Being focusing by first looking with one eye through the eyepiece without the diopter. Close your
other eye. Use
the coarse focusing knob to adjust the height of the stage until the sample comes
into clear focus.
Note: You may loosen the limit-stop screw (located rear of the mechanical stage) in order to give
yourself a larger
range of motion for fine tuning the focus.
4.
Once the image is clear in your field of view, you will want to use the fine focusing knob to tune it for
best results.
Note: Please be careful when moving the mechanical stage if you need to re-center the sample,
or if moving the
stage very close to the objectives. The limit stop is designed to prevent impact
between objective and slide, so when it is off you will be able to damage the microscope. For
safety, when using the 40x and 100x objectives, engage the
limit stop once you have it close or in
contact with the objective (if using oil contact is required).

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